An ultra scale-down analysis of the recovery by dead-end centrifugation of human cells for therapy.

An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions. The key variables studied are the cell suspension hold time prior to centrifugation, the relative centrifugal force (RCF), time of centrifugation, cell pellet resuspension velocities, and number of resuspension passes. The cell critical quality attributes studied are the cell membrane integrity and the presence of selected surface markers. Greater hold times and higher RCF values for longer spin times all led to the increased loss of cell membrane integrity. However, this loss was found to occur during intense cell resuspension rather than the preceding centrifugation stage. Controlled resuspension at low stress conditions below a possible critical stress point led to essentially complete cell recovery even at conditions of extreme centrifugation (e.g., RCF of 10000 g for 30 mins) and long (~2 h) holding times before centrifugation. The susceptibility to cell loss during resuspension under conditions of high stress depended on cell type and the age of cells before centrifugation and the level of matrix crosslinking within the cell pellet as determined by the presence of detachment enzymes or possibly the nature of the resuspension medium. Changes in cell surface markers were significant in some cases but to a lower extent than loss of cell membrane integrity. Biotechnol. Bioeng. 2015;112: 997-1011. © 2014 Wiley Periodicals, Inc

Towards a unified understanding of event-related changes in the EEG:the Firefly model of synchronization through cross-frequency phase modulation

Although event-related potentials (ERPs) are widely used to study sensory, perceptual and cognitive processes, it remains unknown whether they are phase-locked signals superimposed upon the ongoing electroencephalogram (EEG) or result from phase-alignment of the EEG. Previous attempts to discriminate between these hypotheses have been unsuccessful but here a new test is presented based on the prediction that ERPs generated by phase-alignment will be associated with event-related changes in frequency whereas evoked-ERPs will not. Using empirical mode decomposition (EMD), which allows measurement of narrow-band changes in the EEG without predefining frequency bands, evidence was found for transient frequency slowing in recognition memory ERPs but not in simulated data derived from the evoked model. Furthermore, the timing of phase-alignment was frequency dependent with the earliest alignment occurring at high frequencies. Based on these findings, the Firefly model was developed, which proposes that both evoked and induced power changes derive from frequency-dependent phase-alignment of the ongoing EEG. Simulated data derived from the Firefly model provided a close match with empirical data and the model was able to account for i) the shape and timing of ERPs at different scalp sites, ii) the event-related desynchronization in alpha and synchronization in theta, and iii) changes in the power density spectrum from the pre-stimulus baseline to the post-stimulus period. The Firefly Model, therefore, provides not only a unifying account of event-related changes in the EEG but also a possible mechanism for cross-frequency information processing

KEY FACTORS CONTRIBUTING TO COW/CALF COSTS, PROFITS AND PRODUCTION

In this study, cow/calf Standardized Performance Analysis (SPA) data for Texas, Oklahoma, and New Mexico are used to analyze how total cost, production, and profitability are affected by management choices. Total cost is the financial cost associated with raising a calf through the weaning stage; profits are measured using the rate of return on assets; production is determined by pounds weaned per exposed female. Variables such as herd size, pounds of feed fed, calving percentage, death loss, length of breeding season and investment in asset groups are used in regressions. Key factors contributing to a cow/calf operation's costs, production, and profitability are identified.Livestock Production/Industries,

Cosmopolitan Urbanism and Architecture & Paradigm Change in Research and Education in in Asia

The genesis of human settlements is a continuous process of production and layering of spaces in different scale levels across historical periods. The architectural production in our Asian context is always cosmopolitan, hybrid and eclectic, due to historical links and inclusive cultural attitude. Our common building typologies – and also our modernization processes - are the product of the cosmopolitan communities, the articulation of the multi-layered tangible and intangible traditions, and the direct response to local micro-climate of this region. Diversity, eclecticism, fusion, acculturation, adaptation, can perhaps best describe the nature of our architecture and urbanism. Our architectural education now is in dire needs to develop a design-research culture to deal with social and environmental changes. Contemporarily we are in an urgent need to find resolutions to address serious problems posed by the climate change, ideological conflicts, economic greed, depletion of resources, and social justice. Research in architecture should be based on the reality on the ground and not just based on alien theories. Design should be based on the understanding of the real contextual problems, to formulate sensible, sensitive, and holistic solutions. Learning the lessons and wisdoms from our cosmopolitan architecture and urbanism is an important and necessary step towards the improvements and paradigm change in architectural education, research, and practice in Asian context

Rhythms of Consciousness: Binocular Rivalry Reveals Large-Scale Oscillatory Network Dynamics Mediating Visual Perception

Consciousness has been proposed to emerge from functionally integrated large-scale ensembles of gamma-synchronous neural populations that form and dissolve at a frequency in the theta band. We propose that discrete moments of perceptual experience are implemented by transient gamma-band synchronization of relevant cortical regions, and that disintegration and reintegration of these assemblies is time-locked to ongoing theta oscillations. In support of this hypothesis we provide evidence that (1) perceptual switching during binocular rivalry is time-locked to gamma-band synchronizations which recur at a theta rate, indicating that the onset of new conscious percepts coincides with the emergence of a new gamma-synchronous assembly that is locked to an ongoing theta rhythm; (2) localization of the generators of these gamma rhythms reveals recurrent prefrontal and parietal sources; (3) theta modulation of gamma-band synchronization is observed between and within the activated brain regions. These results suggest that ongoing theta-modulated-gamma mechanisms periodically reintegrate a large-scale prefrontal-parietal network critical for perceptual experience. Moreover, activation and network inclusion of inferior temporal cortex and motor cortex uniquely occurs on the cycle immediately preceding responses signaling perceptual switching. This suggests that the essential prefrontal-parietal oscillatory network is expanded to include additional cortical regions relevant to tasks and perceptions furnishing consciousness at that moment, in this case image processing and response initiation, and that these activations occur within a time frame consistent with the notion that conscious processes directly affect behaviour